Triggerd by Offset, Multiplexed, Accurate mass, High-resolution, Absolute Quantification
Mass spectrometry-based assays for protein measurements commonly monitor peptide surrogates, which are easily multiplexed to target many peptides in a single assay. However, these assays have not leveraged the full sample multiplexing capabilities available to shotgun proteomics. TOMAHAQ, represents a two-dimensional multiplexing workflow that relies on synthetic triggering peptides for each protein to prompt the simultaneous quantification of >100 peptides from up to 10 mixed sample conditions.

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